SABOURAUD DEXTROSE AGAR

Technical Data #1015a / 2013.09.17

Sabouraud Dextrose Agar is a solid medium used for isolation, cultivation and maintenance of pathogenic and non pathogenic yeasts and molds. Carbohydrate is an enrichment and low pH inhibits bacterial growth.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

5,0

Meat Peptone

5,0

Dextrose

40,0

Agar

17,0

 

pH 5.6 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 67 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 118 C for 15 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates and streak for isolated colonies. Cutaneous specimens should be press lightly into medium. Inoculate each specimen in duplicate.
  2. Seal plates with masking tape, incubate a set at 35 C for 7 days and the other set, at room temperature for up to 21 days. Tubes should be incubated caps loosened.
  3. Examine frequently every day or every 2-3 days for growth.


QUALITY CONTROL

Results after 7 days at 25C

Organisms

ATCC

Growth

Candida albicans

60193

+

Trichophyton mentagrophytes

9533

+


LIMITATIONS OF METHOD
This medium is only a part of the identification.
Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.:American society for Microbiology.
  2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  3. NCCLS 1990 Quality Assurance for Commercially Prepared Microbiological Culture Mediaicrobiology. Approved Standard Document M22-A Vol.10, No.14


CATALOGUE NUMBERS

Dehydrated media

500 g

QB-39-3806

 

 

 

Prepared media

 

 

Plates, 100x15 mm

10/pkg

1015

Mono-Plates, 100x15 mm

10/pkg

1015M

Petri 100x15 mm, 27ml

10/pkg

1288

Tubes, 16x125 mm

100/box

2487

Tubes, 20x125 mm

10/pkg

2345

Bottles, 2 oz

10/pkg

5060

 


 

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Fax

Internet

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http://www.quelab.com

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1 (800) 579-8177

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