T.C.B.S. (THIOSULFATE CITRATE BILE SALTS SUCROSE) AGAR

Technical Data #1022a / 2013.04.22

T.C.B.S. Agar is a selective solid medium for isolation and cultivation of Vibrio cholerae and other pathogenic vibrios. Gram-positive bacteria are inhibited by the oxbile; sodium thiosulfate and ferric citrate detect H2S production; bromthymol blue and thymol blue are the pH indicators.

 


 

FORMULA
in grams per liter purified filtered water

Yeast Extract

5,0

Casein Peptone

5,0

Meat Peptone

5,0

Sodium Citrate

10,0

Sodium Thiosulfate

10,0

Oxbile

5,0

Sodium Cholate

3,0

Sucrose

20,0

Sodium Chloride

10,0

Ferric Citrate

1,0

Bromthymol Blue

0,04

Thymol Blue

0,04

Agar

14,0

pH 8,6 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 88 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. DO NOT AUTOCLAVE. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plate as soon as possible with specimen and streak for isolation. Swab containing specimen material should be transported to the laboratory in Enteric Pathogen Transport Medium as EPT-100 if inoculation will be delayed.
  2. Incubate at 35 C for 24-48 hours.


QUALITY CONTROL

Results after 24 hrs at 35C

Organisms

ATCC

Growth

Colour

Escherichia coli

25922

- or partial

colorless

Proteus mirabilis

12453

- or partial

yellow

Enterococcus faecalis

29212

- or partial

colorless

Vibrio parahaemolyticus

17802

+

blue

Vibrio fluvialis

clinical strain

+

yellow

Vibrio alginolyticus

clinical strain

+

yellow


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F., 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol.1. Baltimore, MD.: Williams & Wilkins.
  3. Morris, G.K., W.E. DeWitt, E.J. Gangarosa, W.M. McCormack 1976 Enhancement by sodium chloride of the selectivity of Thiosulfate-Citrate-Bile Salts-Sucrose Agar for isolating Vibrio cholerae. J. Clin. Microbiol. 4:133.
  4. West, P.A., E. Russek, P.R. Brayton, P.R. Colwell 1982 Statistical evaluation of a quality control method for isolation of pathogenic Vibrio species on selected Thiosulfate-Citrate-Bile Salts-Sucrose Agars. J. Clin. Microbiol. 16:1110-1116.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-4410
     
Prepared media    
Plates, 100x15 mm 10/pkg 1022
Mono-Plates, 100x15 mm 10/pkg 1022M
Tubes, 16x125 mm 100/box 2924-H
Tubes, 20x125 mm 10/pkg 2345
Bottles, 2 oz 10/pkg 5060

 


 





 

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