SABOURAUD DEXTROSE AGAR, EMMONS

Technical Data #1041a / 2013.04.22

Sabouraud Dextrose Agar, Emmons Modified, is a solid medium used for isolation, cultivation and maintenance of pathogenic and non pathogenic yeasts and molds. Dextrose is decreased to permit a subcultured growth without morphology change. Neutral pH enhances some pathogenic fungi.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

5,0

Meat Peptone

5,0

Dextrose

20,0

Agar

17,0

pH 6,9 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 47 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 118 C for 15 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes..


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates and streak for isolated colonies. Cutaneous specimens should be pressed lightly into medium. Inoculate each specimen in duplicate.
  2. Seal plates with masking tape, incubate a set at 35 C for 7 days and the other set, at room temperature for up to 4-6 weeks. Tubes should be incubated caps loosened.
  3. Examine frequently every day or every 2-3 days for growth..


QUALITY CONTROL

Results after 7 days at 25C

Organisms

ATCC

Growth

Candida albicans

60193

+

Trichophyton mentagrophytes

9533

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Emmons, C.W., C.H. Binford, J.P. Utz, K.H. Kwon-Chung 1977 Medical Mycology. Lea and Febiger, Philadelphia
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  4. NCCLS 1990 Quality Assurance for Commercially Prepared Microbiological Culture Mediaicrobiology. Approved Standard Document M22-A Vol.10, No.14


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3808
     
Prepared media    
Plates, 100x15 mm 10/pkg 1041
Mono-Plates, 100x15 mm 10/pkg 1041M
Tubes, 20x125 mm 10/box 2041
Bottles, 2 oz 20/pkg 5030

 


 





 

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