Technical Data #1041a / 2013.04.22
Sabouraud Dextrose Agar, Emmons Modified, is a solid medium used for isolation, cultivation and maintenance of pathogenic and non pathogenic yeasts and molds. Dextrose is decreased to permit a subcultured growth without morphology change. Neutral pH enhances some pathogenic fungi.
in grams per liter purified filtered water
pH 6,9 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 47 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 118º C for 15 minutes. Cool to 45-50º C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes..
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 7 days at 25ºC
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 g||QB-39-3808|
|Plates, 100x15 mm||10/pkg||1041|
|Mono-Plates, 100x15 mm||10/pkg||1041M|
|Tubes, 20x125 mm||10/box||2041|
|Bottles, 2 oz||20/pkg||5030|
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