POTATO FLAKES AGAR

Technical Data #1081a / 2013.04.22

Potato Flakes Agar is a selective medium used for cultivation of large variety of yeasts and molds.

 


 

FORMULA
in grams per liter purified filtered water

Potato Flakes

40,0

Agar

15,0

pH 5,8 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration).


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates as soon as possible with specimen and streak for isolation.
  2. Incubate at 30 or 35 C as required for 2-7 days.
  3. Examine for growth.


QUALITY CONTROL

Results after 7 days at 30C

Organisms

ATCC

Growth

Candida albicans

10213

+

Candida albicans

60193

+

Trichophyton mentagrophytes

9533

+

Trichophyton rubrum

 

+

Staphylococcus aureus

25923

- or partial


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. Mac Faddin, Jean. F., 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. 1. Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Prepared media    
Plates, 100x15 mm 10/pkg 1081
Mono-Plates, 100x15 mm 10/pkg 1081M

 


 

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