TRYPTIC SOY AGAR (TSA)

Technical Data #1094a / 2013.04.22

Tryptic Soy Agar is a general purpose culture medium for cultivation, isolation of fastidious or nonfastidious microorganisms or for maintenance of stock culture. As it does not contain the X and V factors, it is suitable for identification of Haemophilus sp. by adding X and V factors strips.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

15,0

Soy Peptone

5,0

Sodium Chloride

5,0

Agar

15,0

pH 7,3 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 40 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plate as soon as possible with specimen and streak for isolation. Inoculate tubes with 2-3 similar colonies
  2. Incubate plates at 35 C, under CO2 conditions if necessary, for 24-48 hours. Incubate tubes at 35 C for 24 hours
  3. Examine for growth.


QUALITY CONTROL

Results after 24 hrs at 35C or 60C

Organisms

ATCC

Growth

Streptococcus pyogenes

19615

+

Staphylococcus aureus

25923

+

Escherichia coli

25922

+

Candida albicans

10231

+

Bacillus subtilis

6633

+

Bacillus stearothermophilus

7953

+

Clostridium sporogenes

11437

+

Haemophilus influenzae

10211

+ w/ X and V


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. N.C.C.L.S. 1990 Quality Assurance for Commercially Prepared Microbiological Culture Media. Approved Standard. Vol.10, No.14 NCCLS Document M22-A.
  3. Mac Faddin, Jean. F., 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. 1. Baltimore, MD.: Williams & Wilkins.
  4. Clesceri, L.S., A.E. Greenberg, and R.R. Trussell. 1989 Standard Methods for the Examination of Water and Wastewater. 17th ed. American Public Health Association, Washington, D.C.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-5106
     
Prepared media    
Plates, 100x15 mm 10/pkg 1094
Mono-Plates, 100x15 mm 10/pkg 1094M
Plates, 150x15 mm 5/pkg 1750
Tubes, 20x125 mm 10/pkg 2145
Tubes, 16x125 mm 10/pkg 2859

 


 





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