PSEUDOMONAS P AGAR

Technical Data #1140a / 2013.04.30

Pseudomonas P Agar is a solid medium used for the detection and differentiation of Pseudomonas on the basis of pyocyanin production. Magnesium chloride and potassium sulfate stimulate stimulate pyocyanin production.

 


 

FORMULA
in grams per liter purified filtered water

Gelatin Peptone

20,0

Magnesium Chloride

1,4

Potassium Sulfate

10,0

Agar

15,0

Glycerol

10 ml

pH 7.2 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 47 g of dehydrated media in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Add 10 ml of Glycerol (#8467). Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Inoculate plates with a pure 24 hours culture.
  2. Incubate at 35 C for 24 hours.
  3. Reincubate for additionnal 24 hours at room temperature if no growth.

    Positive result: blue-colored pigment


QUALITY CONTROL

Results after 24 hrs at 35C

Organisms

ATCC

Pyocyanin

Pseudomonas aeruginosa

27853

+

Pseudomonas putida

12633

-

Pseudomonas cepaciae

25416

-


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. King, E.O., M.K. Ward, D.E. Raney. 1954 Two simple media for the demonstration of pyocyanin and flurescein. J. Lab. Clin. Med. 44:301-307
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  4. Washington, J.A. Laboratory Procedures in Clinical Microbiology 1981. Springer-Verlag, New York.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3621
     
Glycerol 100 ml 8467
     
Prepared media    
Plates, 100x15 mm 10/pkg 1140
Mono-Plates, 100x15 mm 10/pkg 1140M

 


 





2325, Dandurand, Suite 300 Phone Fax Internet
Montreal (Quebec) (514) 277-2558 (514) 277-4714 http://www.quelab.com
Canada H2G 1Z9 1 (800) 579-0998 1 (800) 579-8177 info@quelab.com