P.E.A. (PHENYLETHYL ALCOHOL) AGAR
W/ 5% SHEEP BLOOD

Technical Data #1145a / 2013.04.30

P.E.A Agar w/ 5% sheep blood is a selective solid medium used for cultivation and isolation of gram positive cocci from clinical specimens contaminated with mixed flora. This medium should not be used for determination of hemolytic reactions even if sheep blood is added.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

15,0

Soy Peptone

5,0

Sodium Chloride

5,0

Phenylethyl Alcohol

2,5

Agar

15,0

Defibrinated Sheep Blood

50 ml

pH 7.3 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-8 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 42,5 g of dehydrated media in 950 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C and aseptically, add 50 ml of Defibrinated Sheep Blood (4245). Mix gently and dispense into sterile Petri dishes tubes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates as soon as possible with specimen and streak for isolation.
  2. Incubate at 35 C under CO2 conditions for 24-48 hours.
  3. Examine for growth.


QUALITY CONTROL

Results after 24 hrs at 35C W/ CO2

Organisms

ATCC

Growth

Streptococcus pyogenes

19615

+

Streptococcus pneumoniae

6305

+

Staphylococcus aureus

25923

+

Proteus mirabilis

12453

- or partial


LIMITATIONS OF METHOD
For hemolysis activities, a general purpose blood plate (Columbia or TSA with sheep blood) should be inoculated and incubated at the same moment. This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. Liley, B.D., J.H. Brewer 1953 The selective antibacterial action of phenylethyl alcohol. J. Am. Pharm, Assoc. 42:6-8
  3. N.C.C.L.S. 1990 Quality Assurance for Commercially Prepared Microbiological Culture Media. Approved Standard. Vol.10, No.14 NCCLS Document M22-A.
  4. Mac Faddin, Jean. F., 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. 1. Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3521
     
Prepared media    
Plates, 100x15 mm 10/pkg 1145
Mono-Plates, 100x15 mm 10/pkg 1145M
Defibrinated Sheep Blood 50 ml 4245

 


 

 

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