B.H.I.A. (BRAIN HEART INFUSION AGAR)

Technical Data #1177a / 2013.04.30

B.H.I.A. is a general purpose medium for cultivation of large variety of non-fastidious microorganisms including yeasts and molds.

 


 

FORMULA
in grams per liter purified filtered water

Brain Heart Infusion

10,0

Casein Peptone

8,5

Meat Peptone

8,5

Sodium Chloride

5,0

Dextrose

2,5

Disodium Phosphate

2,5

Agar

15,0

pH 7,4 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 52 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates as soon as possible with specimen and streak for isolation.
  2. Incubate at 25 or 35 C as required for 2-7 days.
  3. Examine for growth.


QUALITY CONTROL

Results after 24 hrs at 35C

Organisms

ATCC

Growth

Streptococcus pyogenes

19615

+

Candida albicans

10213

+

Candida albicans

60193

+

Shigella flexneri

12022

+


LIMITATIONS OF METHOD
A too heavy inoculum may result in a light yellow color without affecting the color of the medium and should be considered as a negative result. This is only a part of the identification. Others tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. Finegold, S.M., E.J. Baron 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby, St. Louis
  3. Mac Faddin, Jean. F., 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. 1. Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-0206
     
Prepared media    
Plates, 100x15 mm 10/pkg 1177
Mono-Plates, 100x15 mm 10/pkg 1177M
Tubes, 16x125 mm 10/pkg 2468
Tubes, 20x125 mm 10/pkg 2852

 


 

 

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