m-ENTEROCOCCUS AGAR

Technical Data #1204a / 2013.06.22

m-Enterococcus Agar is a selective medium used in membrane filtration technique or in direct plating for the detection of enterococci in water, foods, feces and other materials.
Sodium azide inhibits gram-negative organisms and enhances the recovery of enterococci. The indicator TTC (triphenyltetrazolium chloride) gives a reddish colour to the colonies of enterococci.

 


 

FORMULA
in grams per liter purified filtered water

Yeast Extract

5,0

Casein Peptone

15,0

Soy Peptone

5,0

Dextrose

2,0

Monopotassium Phosphate

4,0

Sodium Azide

0,4

Triphenyltetrazolium Chloride (TTC)

0,1

Agar

10,0

pH 7,3 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated power, in a dry place, in tightly-sealed containers.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 41 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. DO NOT STERILIZE. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Inoculate surfaces of the plate with membrane filter or inoculate plates with a loopful of specimen and streak for isolation.
  2. Incubate at 35 C for 24-48 hours.


QUALITY CONTROL

Results after 24 hrs at 35C

Organisms

ATCC

Growth

Color

Enterococcus faecalis

29212

+

reddish

Streptococcus pyogenes

19615

-

 

Escherichia coli

25922

-

 


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Mac Faddin, J. 1985 Media for isolation-cultivation-identification-maintenance of medical bacteria.Williams & Wilkins, Baltimore
  2. Rose, R.E., W. Litsky 1965 Enrichment procedure for use with membrane filter for the isolation and enumeration of fecal streptococci. Appl. Microbiol. 13:1-106
  3. Standards Methods for Examination of Water and Wastewater 1989 17th ed. APHA, Washington D.C.


CATALOGUE NUMBERS

Dehydrated media 500 grams QB-39-2695
Prepared media    
Plates,100x15 mm 10/pkg 1204
Mono-Plates, 100x15 mm 10/pkg 1204M

 


 

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