Technical Data #1233a / 2013.01.18
DTM Agar is a solid selective medium for isolation of pathogenic dermatophytic fungi from hair, skin and nails. Antibiotics inhibit bacteria and common molds. Pathogenic dermatophytic fungi produce alkaline reactions changing phenol red, pH indicator, from orange to red.
in grams per liter purified filtered water
pH 5,5 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for laboratory use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 40 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Aseptically add antibiotics as required, mix gently and dispense into sterile Petri dishes or into steriles culture tubes.
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 7 days at 25ºC
- /partial, reddish
LIMITATIONS OF METHOD
A positive reaction in color may occur if specimens are higly contaminated. For this reason, a positive reaction after 10 days should be considered as negative. This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 g||QB-39-3021|
|Mycobiotic/DTM Bi-Plates, 100x15 mm||10/pkg||1233M|
|Tubes, 20x125 mm||10/pkg||2766|
|2325, Dandurand, Suite 300||Phone||Fax||Internet|
|Montreal (Quebec)||(514) 277-2558||(514) 277-4714||http://www.quelab.com|
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