Technical Data #1251a / 2013.05.01
B.C.Y.E. Agar is a solid selective medium used for the isolation and identification of Legionella pneumophila and other Legionella sp. from clinical specimens and environmental samples. L-Cysteine, ferric pyrophosphate, alpha-ketoglutarate and yeast extract are the growth factors.
in grams per liter purified filtered water
pH 6,9 +/- 0,1 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 37 g of dehydrated media in 1000 ml of purified filtered water. Do not heat prior to sterilization. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Aseptically add 2x5 ml of Legionella Supplement (8708), adjust pH with sterile solution of KOH 1N. Mix gently and dispense into sterile Petri dishes.
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 72 hrs at 35ºC under humid atmosphere
Colonies of L. pneumophila are gray-white on this medium and give yellow-green fluorescence under UV light.
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 g||QB-39-2420|
|Plates, 100x15 mm||10/pkg||1251|
|Mono-Plates, 100x15 mm||10/pkg||1251M|
|Legionella Supplement, 5 ml||10/pkg||8708|
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