Technical Data #1268a / 2013.05.01
E.M.B. Agar is a solid medium for isolation and identification of gram-negative enteric bacteria in clinical and non clinical specimens. Addition of sucrose differentiate sucrose fermenters from sucrose non fermenters. Eosin Y and Methylene Blue dyes inhibit a large quantity of gram-positive and stain colonies of lactose and sucrose fermenters. Colonies with metallic sheen confirm Escherichia coli growth. Fecal streptococci will grow on this medium and form pinpoint colonies.
FORMULA
in grams per liter purified filtered water
Gelatin Peptone |
10,0 |
Lactose |
5,0 |
Sucrose |
5,0 |
Dipotassium Phosphate |
2,0 |
Eosin Y |
0,4 |
Methylene Blue |
0,065 |
Agar |
13,5 |
pH 7,2 +/- 0,2 à 25º C |
This approximate formula may be adjusted and/or enriched to obtain best results.
PRECAUTIONS
This medium is for IN VITRO diagnostic use only.
STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder,
in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not
be used if there are signs of contamination or deterioration (shrinking, cracking,
evaporation or discoloration). Do not use dehydrated media if it is caked.
DIRECTIONS
Suspend 36 g of dehydrated media in 1000 ml of purified filtered water. Heat with
frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes.
Cool to 45-50º C. Mix gently and dispense into sterile Petri dishes.
PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.
QUALITY CONTROL
Results after 24 hrs at 35ºC
Organisms |
ATCC |
Growth |
Colour |
Escherichia coli |
25922 |
+ |
black/metallic sheen |
Salmonella typhimurium |
14028 |
+ |
colourless |
Pseudomonas aeruginosa |
27853 |
+ |
colourless |
Enterobacter aerogenes |
13048 |
+ |
brownish |
Streptococcus faecalis |
29212 |
- or partial |
|
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
REFERENCES
CATALOGUE NUMBERS
Dehydrated media | 500 g | QB-39-1606 |
Prepared media | ||
Plates, 100x15 mm | 10/pkg | 1268 |
Mono-Plates, 100x15 mm | 10/pkg | 1268M |
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