HAEMOPHILUS IDENTIFICATION QUAD PLATE

Technical Data #1274a / 2013.05.01

The Haemophilus ID Quad Plate, is used for pathogenic Haemophilus sp. differentiation. Factors X and V are the growth factors and blood will show hemolytic reaction.

 


 

FORMULA
in grams per liter purified filtered water

Special Mixed Peptone

20,0

Sodium Chloride

5,0

Agar

15,0

QUAD I: Factor X

0,01

QUAD II: Factor V

0,01

QUAD III: Factor X and V

0,02

QUAD IV: Factor V

0,01

Defibrinated Horse Blood

50 ml

pH 7,3 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration).


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Suspend 2-3 similar colonies from a Chocolate Bacitracin Agar into a B.H.I. Broth
  2. Inoculate each quadrant with this dilution and streak to obtain isolated colonies. Stab into the blood agar in 4th quadrant.
  3. Incubate at 35 C with 5% CO2 atmosphere for 24 hours.


QUALITY CONTROL

Results after 24 hrs at 35C W/ CO2

Organisms

ATCC

QD I

QD II QD III QD IV
Haemophilus influenzae

10211

-

-

+

+

Haemophilus influenzae

clinical

-

-

+

+

Haemophilus parainfluenzae

clinical

-

+

+

+

Haemophilus haemolyticum

33390

-

-

+

+Hemolysis


LIMITATIONS OF METHOD
Some X dependant microorganisms may carry over factor X to a quadrant without it and causing a false positive growth. This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Finegold, S.M., and W.J. Martin., 1984 Bailey's and Scott's Diagnostic Microbiology. 6th ed. St. Louis. The C.V. Mosby Company.


CATALOGUE NUMBERS

Prepared media    
Mono-Plates, 100x15 mm 10/pkg 1274M

 


 

 

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