N.Y.C. (NEW YORK CITY) MODIFIED AGAR

Technical Data #1276a / 2013.05.04

N.Y.C. Modified Agar is an enriched selective medium used for the isolation and cultivation of pathogenics Neisseria sp. Lysed horse blood, horse serum, dextrose and yeast dialysate are the enrichments which permit a luxuriant recovery of pathogenic Neisseria sp. Vancomycin, a gram-positive cocci inhibitor, is replaced by lincomycin to prevent inhibition of some strains of Neisseria gonorrheae. Colimycin inhibits gram-negative bacilli, amphotericin B suppress yeast growth and trimethoprim lactate prevents Proteus sp. swarming.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

7,5

Meat Peptone

7,5

Corn Starch

1,0

Potassium Phosphate, dibasic

4,0

Potassium Phosphate, monobasic

1,0

Sodium Chloride

5,0

Agar

10,0

Enrichments

 

Dextrose 50%

10 ml

Yeast Dialysate

25 ml

Horse Serum

120 ml

Lysed Horse Blood

75 ml

Antibiotics

 

L.C.A.T

10 ml

pH 7,4 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 36 g of dehydrated media in 750 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Add Dextrose 50% (8580), 25 ml of Yeast Dialysate (8643), 120 ml of Horse Serum (4602), 75 ml of Lysed Horse Blood (4177) and 10 ml of LCAT (8610). Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plate as soon as possible with specimen and streak for isolation.
  2. Incubate at 35 C with 5% CO2 for 48 hours.


QUALITY CONTROL

Results after 48 hrs at 35C w/ CO2

Organisms

ATCC

Growth

Neisseria gonorrheae

43069

+

Neisseria gonorrheae

clinical

+

Neisseria meningitidis

13090

+

Proteus mirabilis

43071

- or partial

Escherichia coli

25922

- or partial

Neisseria sicca

9913

-

Candida albicans

60193

- or partial

Staphylococcus epidermidis

12228

- or partial


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Faur, Y.C., M.H. Weisburd, M.E. Wilson 1978 The selectivity of vancomycin and lincomycin in NYC medium for the recovery of N. gonorrheae from clinical specimens. Health Lab. Sci. 15:22-27
  2. Granato, P.A., C. Schneible-Smith, L. B. Weiner 1981 Use of New York City Medium for improved recovery of Neisseria gonorrheae from clinical specimens 13:963-968
  3. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-1906
     
Prepared media    
Plates, 100x15 mm 10/pkg 1276
Mono-Plates, 100x15 mm 10/pkg 1276M
Jembec NYC modified, rectangular 10/pkg 1410
     
Dextrose 50% 100 ml 8580
Yeast Dialysate 25 ml 8643
Horse Serum 150 ml 4602
Lysed Horse Blood 100 ml 4177
LCAT Supplement, 10 ml 10/pkg 8610

 


 

 

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