Technical Data #1334a / 2013.06.27
Mueller Hinton Agar with 5% sheep blood is a solid medium used for susceptibility testing of streptococci. Bauer et al. have recommanded this medium as routine methods for disk susceptibility testing. This medium is not recommanded for sulfonamide and trimethoprim testing. Blood components inhibit clear diffusion of these antibiotics.
in grams per liter purified filtered water
|Acid Casein Hydrolysate||
|Defibrinated Sheep Blood||
pH 7,3 +/- 0,1 à 25° C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 38 g of Mueller Hinton dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121° C for 15 minutes. Cool to 45-50° C. Add 50 ml of defibrinated sheep blood (4245). Mix gently and dispense into sterile Petri dishes.
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 24 hrs at 35ºC w/ CO2
meets NCCLS Standards
LIMITATIONS OF METHOD
Lysed Blood should replace fresh blood if sulfonamide and trimethoprim susceptibility testing are required. This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 grams||QB-39-3206|
|Plates, 100x15 mm||10/pkg||1334|
|Plates, 150x15 mm||5/pkg||1735|
|Mueller Hinton Broth|
|Tubes 16x125 mm||10/pkg||2663|
|Defibrinated Sheep Blood||4245|
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