NUTRIENT AGAR

Technical Data #1369a / 2013.09.26

Nutrient Agar is a solid general purpose medium used for the cultivation of non fastidious microorganisms. This medium is used in the examination of water and wastewater, dairy product and foods.

 


 

FORMULA
in grams per liter purified filtered water

Gelatin Peptone

5,0

Beef Extract

3,0

Agar

15,0

pH 6,8 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 23 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes or into sterile culture tubes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates and streak for isolated colonies.
  2. Tubes used for maintenance of pure culture, should be inoculated with a single colony.
  3. Incubate at 35 C for 24 hours.


QUALITY CONTROL

Results after 24 hrs at 35C

Organisms

ATCC

Growth

Staphylococcus aureus

25923

+

Escherichia coli

25922

+

Corynebacterium diphteriae

 

+

Pseudomonas aeruginosa

27853

+


LIMITATIONS OF METHOD
This is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  3. Standards Methods for Examination of Water and Wastewater 1989 17th ed. APHA, Washington D.C.


CATALOGUE NUMBERS

Dehydrated media 500 grams QB-39-3406
     
Prepared media    
Plates, 100x15 mm 10/pkg 1369
Mono-Plates, 100x15 mm 10/pkg 1369M
Tubes, 16x125 mm 10/pkg 2741
Mini-Vial, 17x55 mm 100/box 2565

 


 

 

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