PLATE COUNT AGAR

Technical Data #1379a / 2013.07.03

Plate Count Agar is a solid medium recommended by APHA and AOAC for microbial plate counts from milk, foods and water.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

5,0

Yeast Extract

2,5

Glucose

1,0

Agar

15,0

pH 7,0 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 23,5 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates following recommended procedures.
  2. Incubate at recommended temperature and for recommended number of days.
  3. Examine frequently for counting of colonies.


QUALITY CONTROL

Results after 24 hrs at 35C

Organisms

ATCC

Growth

Pseudomonas aeruginosa

27853

+

Staphylococcus aureus

25923

+

Escherichia coli

25922

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Greenberg, Trussell and Clesceri 1985 Standards methods for the examination of water and wastewater. 16thed APHA Washington.
  2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  3. Richardson 1985 Standard methods for the examination of dairy products. 15th ed APHA Washington
  4. Speck 1984 Compendium of methods for the microbiological examination of foods 2nd ed APHA Washington.


CATALOGUE NUMBERS

Dehydrated media 500 grams QB-39-4306
Prepared media    
Plates, 100x15 mm 10/pkg 1379
Mono-Plates, 100x15 mm 5/pkg 1379M
Tubes, 20x125 mm 10/pkg 2461

 


 

 

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