Technical Data #1398a / 2013.05.29
Biggy Agar is a selective medium for isolation and presumptive identification of Candida sp. by their ability to reduce sodium sulfite to sulfide giving a brown to black color. Sodium sulfite and bismuth ammonium citrate suppress bacterial growth.
in grams per liter purified filtered water
|Bismuth Ammonium Citrate||
pH 6,8 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 45 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. DO NOT STERILIZE. Cool to 45-50º C. Mix gently and dispense into sterile Petri dishes.
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 3 days at 25ºC
+ black center
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 g||QB-39-0130|
|Plates, 100x15 mm||10/pkg||1398|
|Mono-Plates, 100x15 mm||10/pkg||1398M|
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