Technical Data #1398a / 2013.05.29
Biggy Agar is a selective medium for isolation and presumptive identification of Candida sp. by their ability to reduce sodium sulfite to sulfide giving a brown to black color. Sodium sulfite and bismuth ammonium citrate suppress bacterial growth.
FORMULA
in grams per liter purified filtered water
Yeast Extract |
1,0 |
Glycine |
10,0 |
Dextrose |
10,0 |
Sodium Sulfite |
3,0 |
Bismuth Ammonium Citrate |
5,0 |
Agar |
16,0 |
pH 6,8 +/- 0,2 à 25º C |
This approximate formula may be adjusted and/or enriched to obtain best results.
PRECAUTIONS
This medium is for IN VITRO diagnostic use only.
STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
DIRECTIONS
Suspend 45 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. DO NOT STERILIZE. Cool to 45-50º C. Mix gently and dispense into sterile Petri dishes.
PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.
QUALITY CONTROL
Results after 3 days at 25ºC
Organisms |
ATCC |
Growth |
Candida albicans |
10231 |
+ brown |
Candida tropicalis |
750 |
+ black center |
Escherichia coli |
25922 |
- |
Staphylococcus aureus |
25923 |
- |
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
REFERENCES
CATALOGUE NUMBERS
Dehydrated media | 500 g | QB-39-0130 |
Prepared media | ||
Plates, 100x15 mm | 10/pkg | 1398 |
Mono-Plates, 100x15 mm | 10/pkg | 1398M |
Jembec Kit | 10/pkg | 1354 |
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