BIGGY (BISMUTH SULFITE GLUCOSE GLYCINE YEAST) AGAR

Technical Data #1398a / 2013.05.29

Biggy Agar is a selective medium for isolation and presumptive identification of Candida sp. by their ability to reduce sodium sulfite to sulfide giving a brown to black color. Sodium sulfite and bismuth ammonium citrate suppress bacterial growth.

FORMULA
in grams per liter purified filtered water

Yeast Extract	1,0
Glycine	10,0
Dextrose	10,0
Sodium Sulfite	3,0
Bismuth Ammonium Citrate	5,0
Agar	16,0

pH 6,8 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 45 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. DO NOT STERILIZE. Cool to 45-50º C. Mix gently and dispense into sterile Petri dishes.

PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

1. Inoculate plates with specimen and streak to obtain isolated colonies.
2. Incubate at 25º C for 3 days
3. Examine daily for sulfide reduction.

QUALITY CONTROL
Results after 3 days at 25ºC

Organisms	ATCC	Growth
Candida albicans	10231	+ brown
Candida tropicalis	750	+ black center
Escherichia coli	25922	-
Staphylococcus aureus	25923	-

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Barr, F.S., G.F. Collins 1966 A rapid method for the isolation and identification of Candida. South Med. J. 59:694
2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
3. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
4. Nickerson, W., 1953 Reduction of inorganic substances by yeasts. Extracellular reduction of sulfide by species of Candida. J. Infec. Dis. 93:43

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-0130
Prepared media
Plates, 100x15 mm	10/pkg	1398
Mono-Plates, 100x15 mm	10/pkg	1398M
Jembec Kit	10/pkg	1354