MYGP COPPER AGAR

Technical Data #1405a / 2013.08.18

MYGP Copper Agar is a solid selective medium used for isolation and cultivation of wild yeasts in the brewing industry. The addition of copper inhibits the larger yeasts. The medium is recommended for the microbiological quality of beers in process.

 


 

FORMULA
in grams per liter purified filtered water

Yeast Extract

3,0

Malt Extract

3,0

Gelatin Peptone

5,0

Dextrose

10,0

Cupric Sulfate

0,4

Agar

20,0

pH 6,2 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 41,4 g of dehydrated media in 1000 ml of purified filtered. Heat with frequent agitation and boil for one minute. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates with 100 ul of diluted sample
  2. Distribute inoculum on the surface of the plates
  3. Incubate at 30 C for 3-4 days.


QUALITY CONTROL

Results after 48 hrs at 35C

Organisms

ATCC

Growth

Lactobacillus fermentum

9338

+

Candida albicans

10231

+

Saccharomyces cerevisiae

9763

+

Aspergillus niger

16404

+

Escherichia coli

25922

-


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. American Society of Brewing Chemists. Report of Subcommittee on Copper Media for Wild Yeast Detection. 1992 Journal 50:153
  2. Lee, S., Jangaard, N., Coors, J., Hsu, W., Fuchs, C., and Brenner, M. Proc. Am. Soc. Brew. Chem. 1975 33:18
  3. Taylor, G., and Marsh, A., J. Inst. Brew. 1984 90:135


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3211
     
Prepared media    
Plates, 100x15 mm 10/pkg 1405
Mono-Plates, 100x15 mm 10/pkg 1405M

 


 

 

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