Technical Data #1426a / 2013.08.18
Bacillus Cereus Agar is a solid selective medium for isolation of Bacillus cereus from food in small amount even in presence of large numbers of contaminates. Sodium pyruvate enhances egg yolk precipitation and bromthymol blue is the pH indicator for the mannitol fermentation. Polymixin B is the selective agent.
in grams per liter purified filtered water
|Sodium Phosphate, dibasic||
|Potassium Phosphate, monobasic||
|Egg Yolk Solution||
100 000 units
pH 7,6 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for laboratory use only.
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 41 g of dehydrated media in 950 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121° C for 15 minutes. Cool to 45-50° C. Aseptically add 50 ml of sterile solution of Egg yolk (#8653) and 100 000 units of a sterile solution of Polymixin B. Mix gently and dispense into sterile Petri dishes .
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 48 hrs at 35ºC
+ Greenish-blue w/ halo
+/- w/o halo
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 g||QB-39-0150|
|Egg Yolk Supplement||100 ml||8653|
|Polymixin B||4 MM units||QB-39-3514|
|Plates, 100x15 mm||10/pkg||1426|
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