MALT EXTRACT AGAR

Technical Data #1449a / 2013.07.03

Malt Extract Agar is a solid medium used for isolation, cultivation and maintenance of yeasts and molds. High carbohydrates content enhances the rapid growth of yeasts and molds and low pH inhibits most bacteria.

 


 

FORMULA
in grams per liter purified filtered water

Gelatin Peptone

0,78

Maltose

12,75

Dextrin

2,75

Glycerol

2,35

Agar

15,0

pH 4,6 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 33,6 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 118 C for 15 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates and streak for isolated colonies.
  2. Seal plates with masking tape, incubate at room temperature for 7 days.
  3. Examine frequently every day or every 2-3 days for growth.


QUALITY CONTROL

Results after 7 days at 25C

Organisms

ATCC

Growth

Candida albicans

60193

+

Candida albicans

10231

+

Trichophyton mentagrophytes

9533

+

Aspergillus niger

16404

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 grams QB-39-2810
     
Prepared media    
Plates, 100x15 mm 10/pkg 1449
Mono, 100x15 mm 10/pkg 1449M

 


 





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