Technical Data #1480a / 2013.07.03
Violet Red Bile Agar is a solid medium used for the detection and isolation of coliforms in food, water, milk and sanitary material.
Bile salts and crystal violet are the inhibitors of gram-positive microorganisms. Lactose fermenting coliforms give red colonies with precipitation of bile salts.
FORMULA
in grams per liter purified filtered water
Casein Peptone |
7,0 |
Bile Salts #3 |
1,5 |
Yeast Extract |
3,0 |
Lactose |
10,0 |
Sodium Chloride |
5,0 |
Neutral Red |
0,03 |
Crystal Violet |
0,002 |
Agar |
15,0 |
pH 7,4 +/- 0,2 à 25° C |
This approximate formula may be adjusted and/or enriched to obtain best results.
PRECAUTIONS
This medium is for IN VITRO diagnostic use only.
STORAGE
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
DIRECTIONS
Suspend 42 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121° C for 15 minutes. Cool to 45-50° C. Mix gently and dispense into sterile Petri dishes.
PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.
QUALITY CONTROL
Results after 24 hrs at 35ºC
Organisms |
ATCC |
Growth |
Colour |
Escherichia coli |
25922 |
+ |
red, bile point |
Enterobacter aerogenes |
13048 |
+ |
pinkish |
Salmonella typhimurium |
14028 |
+ |
colorless |
Streptococcus faecalis |
29212 |
- |
|
Staphylococcus aureus |
25923 |
- |
|
LIMITATIONS OF METHOD
Selectivity may decrease after 24 hours incubation. This medium is only a part of the identification. Other tests may be required.
REFERENCES
CATALOGUE NUMBERS
Dehydrated media | 500 grams | QB-39-5601 |
Prepared media | ||
Plates, 100x15 mm | 10/pkg | 1480 |
Mono-Plates, 100x15 mm | 10/pkg | 1480M |
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