M-FC AGAR

Technical Data #1495a / 2013.07.03

m-FC Agar is a selective solid medium for detection and
enumeration of fecal coliforms organisms by the membrane filter
technique. Bile salts inhibit gram-positive bacteria. Fecal coliforms ferment lactose at the elevated temperature. With addition of rosolic acid, coliforms are blue and the other organisms appear grey or cream-colored bacteria.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

9,0

Yeast Extract

6,5

Meat Peptone

2,5

Lactose

12,5

Sodium Chloride

5,0

Bile Salts

1,5

Aniline Blue

0,1

Agar

15,0

pH 7,4 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 52,1 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Add 10 ml of 1% Rosolic Acid Solution. DO NOT AUTOCLAVE. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Place the membrane filter used to collect the water sample, on the surface of the agar. Avoid air bubbles between surface of agar and membrane filter.
  2. Seal individual dish with waterproof tape.
  3. Immerge Petri dishes in a water bath at 44,5 C for 24 hours.
  4. Remove from water bath and count blue colonies.


QUALITY CONTROL

Results after 24 hrs at 44C

Organisms

ATCC

Growth

Colour

Escherichia coli

25922

+

blue

Enterobacter aerogenes

13048

+

grey

Salmonella typhimurium

14028

-

 

Streptococcus faecalis

29212

-

 


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Grabow, W.O.K., C.A. Hilner, C.A. & P. Coubrough. 1981 Evaluation of standard and modified M-FC, Mac Conkey and Teepol media for membrane filter counting of fecal coliform in water. Appl. Environ. Microbiol. 42:192.
  2. Mac Faddin, Jean F., 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol.1. Baltimore, MD.: Williams & Wilkins.
  3. Standard Methods for Examination of Water and Wastewater 1989 17th ed. APHA Washington D.C.


CATALOGUE NUMBERS

Dehydrated media 500 grams QB-39-2908
Prepared media    
Plates, 100x15 mm 10/pkg 1523
Mono-Plates, 100x15 mm 10/pkg 1523M
Plates, 60x15 mm 10/pkg 1623
Plates, 150x15 mm 5/pkg 1495

 


 





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