Technical Data #1528a / 2013.05.20
Oxford Listeria Agar supplemented with antibiotics is used for the selective isolation of Listeria monocytogenes from foods. Esculin hydrolysed by the Listeria sp. strains give black colonies. Antibiotics and lithium chloride are the inhibitors of gram-positve and gram-negative bacteria.
FORMULA
in grams per liter purified filtered water
Casein Peptone |
10,0 |
Meat Peptone |
5,0 |
Heart Peptone |
3,0 |
Yeast Extract |
5,0 |
Sodium Chloride |
5,0 |
Corn Starch |
1,0 |
Esculin |
1,0 |
Ferric Ammonium Citrate |
0,5 |
Lithium Chloride |
15,0 |
Agar |
15,5 |
Supplement |
|
Oxford |
10 ml |
pH 7,2 +/- 0,2 à 25º C |
This approximate formula may be adjusted and/or enriched to obtain best results.
PRECAUTIONS
This medium is for IN VITRO diagnostic use only.
STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
DIRECTIONS
Suspend 61 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Add 10 ml of Oxford Medium Supplement (#8716). Mix thouroughly and dispense into Petri dishes.
PROCEDURE
Bring prepared medium at room temperature before inoculation.
QUALITY CONTROL
Expected results after 24 hrs at 35ºC
Organisms |
ATCC |
Growth |
Listeria monocytogenes |
15313 |
+ black colonies |
Staphylococcus aureus |
25923 |
- or partial |
Escherichia coli |
25922 |
- or partial |
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
REFERENCES
CATALOGUE NUMBERS
Dehydrated media | 500 g | QB-39-3508 |
Oxford Supplement, 10x5 ml | 10/pkg | 8716 |
Modified Oxford Supplement, 10x5 ml | 10/pkg | 8717 |
Prepared media | ||
Plates, 100x15 mm | 10/pkg | 1528 |
Mono-Plates, 100x15 mm | 10/pkg | 1528M |
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