Technical Data #1571a / 2013.05.21
Potato Dextrose Agar is a solid medium used for isolation, cultivation of yeasts and molds and to stimulate sporulation.
in grams per liter purified filtered water
|Potato, Infusion from||
pH 5,6 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 39 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Mix gently and dispense into sterile Petri dishes. To adjust pH at 3,5, add 10 ml of sterile Tartaric Acid Solution at 10% (8591).
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 7 days at 25ºC
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 g||QB-39-3606|
|Plates, 100x15 mm||10/pkg||1571|
|Mono-Plates, 100x15 mm||10/pkg||1571M|
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