INHIBITORY MOLD AGAR (I.M.A.)

Technical Data #1576a / 2014.02.07

Inhibitory Mold Agar is a solid selective medium used for isolation and cultivation of yeasts and pathogenic molds from contaminated specimens. With added ciprofloxacin inhibits bacterial growth.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

3,0

Meat Peptone

2,0

Yeast Extract

5,0

Starch, Soluble

2,0

Dextrin

1,0

Sodium Phosphate

2,0

Magnesium Sulfate

0,8

Iron Sulfate

0,04

Sodium Chloride

0,04

Manganese Sulfate

0,16

Ciprofloxacin

0,005

Agar

15,0

 

pH 6.7 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 36 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Aseptically add 10ml of ciprofloxacin. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates and streak for isolated colonies. Cutaneous specimens should be press lightly into medium. Inoculate each specimen in duplicate.
  2. Seal plates with masking tape, incubate a set at 35 C for 7 days and the other set, at room temperature for up to 4-6 weeks. Tubes should be incubated caps loosened.
  3. Examine weekly for growth.


QUALITY CONTROL

Results after 7 days at 25C

Organisms

ATCC

Growth

Candida albicans

10231

+

Trichophyton mentagrophytes

9533

+

Aspergillus niger

16404

+

Escherichia coli

25922

- or partial


LIMITATIONS OF METHOD
This medium is only a part of the identification.
Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  3. Washington, J.A. Jr., 1981 Laboratory procedures in Clinical Microbiology, Springer-Verlag, New York


CATALOGUE NUMBERS

Dehydrated media

500 g

QB-39-2208

 

 

 

Prepared media

 

 

Plates, 100x15 mm

10/pkg

1576

Mono-Plates, 100x15 mm

10/pkg

1576M

 

 

 

Supplement

 

 

10x5 ml

10/pkg

8840

 


 

 

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