CYCLOSERINE CEFOXITIN AGAR

Technical Data #1587a / 2013.05.21

Cycloserin Cefoxitin Agar is a solid selective medium, sheep blood supplemented, used for isolation and recovery of Clostridium difficile from feces. Cycloserin and cefoxitin, the selective agents, inhibit intestinal normal flora growth.

 


 

FORMULA
in grams per liter purified filtered water

Proteose Peptone #2

40,0

Phosphate disodique

5,0

Phosphate monopotassique

2,0

Magnesium Sulfate

1,0

Sodium Chloride

5,0

Agar

13,5

Supplements

 

Cycloserin

0,5

Cefoxitin

0,016

Defibrinated Sheep Blood

50 ml

pH 7.2 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light. Medium must not be used if there are signs of contamination or if the expiry date has passed.


PROCEDURE
Bring prepared media at room temperature before inoculation

  1. Inoculate specimens without any pre-treatment. Streak to obtain isolated colonies.
  2. Incubate at 35 C under anaerobic atmosphere for 48 hours.


QUALITY CONTROL

Results after 48 hrs at 35C anaerobically

Organisms

ATCC

Growth

Clostridium difficile

9689

+

Escherichia coli

25922

-

Staphylococcus aureus

25923

-

Colonies of Clostridium difficile, are greyish, average of 4 mm- diameter, rounds with filamentous edges, low umbonate. Because of high selectivity of medium, it is recommanded to inoculate also a non-selective anaerobic blood agar and incubate under anaerobic atmosphere.


LIMITATIONS OF METHOD
This is only a part of identification. Other tests may be required.


REFERENCES

  1. George, W.L., V.L. Sutter, D. Citron and S.M. Finegold 1979 Selective and differentiel medium for Isolation of Clostridium difficile. J. Clin. Microbiol. 9:214-219.
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F., 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 Baltimore, MD. Williams & Wilkins.
  4. Willey, S., J.G. Bartlett 1979 Cultures for Clostridium difficile in Stools Containing a Cytotoxin Neutralized by Clostridium sordellii Antitoxin. J. Clin. Microbiol. 10:880-884


CATALOGUE NUMBERS

Prepared media    
Plates, 100x15 mm 10/pkg 1587
Mono-Plates, 100x15 mm 10/pkg 1587M
     
Clostridium Supplement,
10 x 5 ml
Vials 8705
     
Defibrinated Sheep Blood 50 ml 4245

 


 

 

2325, Dandurand, Suite 300 Phone Fax Internet
Montreal (Quebec) (514) 277-2558 (514) 277-4714 http://www.quelab.com
Canada H2G 1Z9 1 (800) 579-0998 1 (800) 579-8177 info@quelab.com