BILE ESCULIN AGAR

Technical Data #1638a / 2013.06.10

Bile Esculin Agar is a selective culture medium used for the isolation of group D streptococci. Esculin hydrolysed by group D enterococci give a dark brown colour. Oxgall inhibits Gram-positive bacteria other than enterococci.

 


 

FORMULA
in grams per liter purified filtered water

Gelatin Peptone

5,0

Beef Extract

3,0

Oxgall

40,0

Esculin

1,0

Ferric Citrate

0,5

Agar

15,0

Horse Serum

50 ml

pH 6,6 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration) Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 64 g in 950 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C and add 50 ml of horse serum (4173). Mix thouroughly and dispense into Petri dishes or into sterile culture tubes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate with 2-3 similar colonies.
  2. Incubate at 35 C for 24 hours.


QUALITY CONTROL

Expected results after 24 hrs at 35C

Organisms

ATCC

Growth

Blackening

Streptococcus faecalis

29212

+

+

Streptococcus pyogenes 19615 - or partial -


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
  3. N.C.C.L.S. 1990 Quality Assurance for Commercially Prepared Microbiological Culture Media Approved Standard. Vol. 10, No. 14, NCCLS Document M22-A


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-1115
     
Prepared media    
Plates, 100x15 mm 10/pkg 1638
Mono-Plates, 100x15 mm 10/pkg 1638M
Tubes 16x125 mm, 5 ml 10/pkg 2540
  100/box 2540-H
     
Horse Serum 50 ml 4173

 


 

 

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