ENDO AGAR

Technical Data #1647a / 2013.08.18

Endo Agar is a differential and slightly selective solid medium for detection of coliforms and other enteric micro-organisms in water, wastewater, dairy products and foods. Sodium sulfite and basic fuchsine inhibit gram-positive bacteria. Coliforms ferment lactose and give pink-reddish colonies.

 


 

FORMULA
in grams per liter purified filtered water

Gelatine Peptone

10,0

Lactose

10,0

Potassium Phosphate, dibasic

3,5

Sodium Sulfite

2,5

Basic Fuchsine

0,5

Agar

15,0

pH 7,5 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 41,5 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute.Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plate as soon as possible with specimen and streak for isolation.
  2. Incubate at 35 C for 24-48 hours.


QUALITY CONTROL

Results after 24 hrs at 35C

Organisms

ATCC

Growth

Colour

Escherichia coli

25922

+

red/metallic sheen

Salmonella typhimurium

14028

+

colorless


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Standard Methods for Examination of Water and Wastewater 1989 17th ed. APHA Washington D.C.
  2. Compendium of methods for the microbiological examination of foods. 2nd, ed APHA Washington, DC


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-1610
     
Prepared media    
Plates, 100x15 mm 10/pkg 1647

 


 

 

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