CAMPYLOBACTER SKIRROW AGAR

Technical Data #1727a / 2013.09.25

Campylobacter Skirrow Agar is a selective solid medium used for the cultivation and isolation of Campylobacter jejuni from fecal specimens. Addition of antibiotics refrains normal stool flora overgrowth and enhances C. jejuni recovery.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

12,0

Meat Peptone

5,0

Yeast Extract

3,5

Beef Extract

3,0

Corn Starch

1,0

Sodium Chloride

5,0

Agar

13,5

Antibiotics

 

Vancomycin

0,010

Trimetoprim lactate

0,005

Amphotericin B

0,002

Polymyxin B

2500 units

Hemolyzed Sheep Blood

150 ml

pH 7.4 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C. Antibiotics supplements (8745) are stable to up one year if stored at -20 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 43 g in 850 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C and aseptically add 150 ml of Hemolyzed Sheep Blood (4379) and 10 ml of Supplements Skirrow (8703). Dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plate as soon as possible with specimen and streak for isolation. If inoculation will be delayed, an Enteric Pathogen Transport Medium as EPT-100 should be used.
  2. Incubate at 42 C under a reduced O2 atmosphere for 48 hours.


QUALITY CONTROL

Results after 48 hrs at 42C

Organisms

ATCC

Growth

Campylobacter jejuni

33291

+

Escherichia coli

25922

- or partial

Proteus mirabilis

12453

- or partial

Streptococcus faecalis

29212

- or partial

Candida albicans

10231

- or partial

Colonies of C. jejuni will appear on Campylobacter Skirrow Agar as small, mucoid, flat or slightly raised, non-hemolytic translucent and gray. Some strains may vary from brown to tan.


LIMITATIONS OF METHOD
Since C. jejuni is thermophilic, plates should be incubated at, at least 42 C. This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Blaser, M.J., I.V. Berkowitz, FM. LaForce, J. Cravens, L.B. Reller, and W-L. L. Wang. 1979 Campylobacter enteritis: clinical and epidimiologic features. Ann. Intern. Med. 91: 179-185.
  2. Karmali, M.A., P.C. Fleming. 1979 Campylobacter enteritidis CMA Journal. 120:1525-1532.
  3. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  4. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  5. N.C.C.L.S. Quality Assurance for Commercially prepared Microbiological Culture Media. N.C.C.L.S. Publication M-22-A Approved Standard 1990 Vol. 10 No.14.
  6. Skirrow, M.B. 1977 Campylobacter enteritidis: a "new" disease. Br. Med. J. 2:9-11.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-1005
     
Skirrow Supplement 10x5 ml 8703
     
Hemolysed Sheep Blood 150 ml 4379
     
Prepared media    
Plates, 100x15 mm 10/pkg 1727
Mono-Plates, 100x15 mm 10/pkg 1727M
     
Enteric Pathogen Transport, Vial 100/cs EPT-100
     
Campylobacter Skirrow Kit 6x1 L. QB-KT-0112

 


 

 

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