UNIVERSAL BEER AGAR WITH CYCLOHEXIMIDE

Technical Data #1732a / 2013.08.18

Universal Beer Agar with Actidione is a solid selective medium used for isolation and cultivation of microorganisms in the brewing industry. The addition of beer inhibits the microorganisms adapted in the brewery and some not adapted in this environmental conditions. The cycloheximide reacts as a fongicide.

 


 

FORMULA
in grams per liter purified filtered water

Yeast Extract

6,1

Peptonized Milk

15,0

Tomato Juice

12,2

Dextrose

16,1

Potassium Phosphate, dibasic

0,31

Potassium Phosphate, monobasic

0,31

Magnesium Sulfate

0,12

Manganese Sulfate

0,006

Sodium Chloride

0,006

Ferrous Sulfate

0,006

Agar

12,0

Antibiotic

 

Cycloheximide

0,01

pH 6,3 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 62,0 g of dehydrated media in 750 ml of purified filtered water containing 3 ml de Tween 80. Add 0,01 g of Cycloheximide. Heat with frequent agitation and boil for one minute. Add 250 ml of not-degassed beer. Sterilize at 121 C for 10 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates and streak for isolated colonies.
  2. Incubate at 30 C for 48-72 hours


QUALITY CONTROL

Results after 48 hrs at 35C

Organisms

ATCC

Growth

Lactobacillus fermentum

9338

+

Saccharomyces cerevisiae

9763

+

Proteus mirabilis

12453

+/-


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Boatwright, J., and Kirsop, B.H., 1976 J. Inst. Brew. 82:343-346
  2. Kozulis and Page 1968 Proc. Am. Soc. Brewing Chemists
  3. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-5300
     
Cycloheximide 10 g QB-60-1015
     
Prepared media    
Plates, 100x15 mm 10/pkg 1732
Mono-Plates, 100x15 mm 10/pkg 1732M
Bouteille, 200 ml 10/pkg 5212

 


 

 

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