Technical Data #1771a / 2013.06.11
Regan Lowe Agar w/o antibiotics is solid medium used for the cultivation and isolation of Bordetella pertussis and Bordetella parapertussis from nasopharyngeal specimens. Nicotinic acid is an essential nutrient for Bordetella growth. Charcoal and starch, as absorbents, remove fatty acids.
in grams per liter purified filtered water
|Defibrinated Horse Blood||
pH 7,4 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8º C protected from direct light. and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 54 g in 900 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C and aseptically add 100 ml of Defibrinated Horse Blood (4281). Dispense into sterile Petri dishes.
Prior to inoculate, the prepared medium should be brought to room temperature.
Expected results after 3 days at 35ºC under humid atmosphere
Colonies of Bordetella pertussis are tiny, smooth, with a characteristic compact, glistening appearance.
LIMITATIONS OF METHOD
Regan-Lowe Agar w/o antibiotics may be used along with selective Regan Lowe Agar. This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 g||QB-39-0911|
|Defibrinated Horse Blood||100 ml||4281|
|Plates, 100x15 mm||10/pkg||1771|
|Mono-plates, 100x15 mm||10/pkg||1771M|
|B. Pertussis Transport Medium, Vial 8 ml||10/pkg||2555|
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