SA.B.H.I. (SABOURAUD BRAIN HEART INFUSION) AGAR

Technical Data #1786a / 2013.08.18

SA. B.H.I. Agar is a general purpose medium for the isolation and cultivation of fungi and dermatophytes from clinical or non clinical specimens.

 


 

FORMULA
in grams per liter purified filtered water

Brain Heart Infusion

10,0

Casein Peptone

2,25

Meat Peptone

7,25

Sodium Chloride

2,5

Dextrose

21,0

Disodium Phosphate

1,25

Agar

15,0

pH 7,0 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 59,3 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates as soon as possible with specimen and streak for isolation. Cutaneous specimens should be press lightly into medium. Inoculate each specimen in duplicate.
  2. Seal plates with masking tape, incubate a set at 35 C for 7 days and the othe set, at room temperature for up to 21 days.
  3. Examine weekly and discard after 4-6 weeks for a negative growth.


QUALITY CONTROL

Results after 7 days at 25C

Organisms

ATCC

Growth

Candida albicans

10213

+

Candida albicans

60193

+

Trichophyton mentagrophytes

9533

+

Aspergillus niger

16404

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. Mac Faddin, Jean. F., 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. 1. Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3830
     
Prepared media    
Plates, 100x15 mm 10/pkg 1786
Mono-Plates, 100x15 mm 10/pkg 1786M

 


 

 

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