Technical Data #1810a / 2013.11.01
Bordet Gengou Agar is used for the primary isolation of Bordetella pertussis and Bordetella parapertussis from clinical specimens. Potato infusion, casein peptone and glycerol are
the essential nutrients for Bordetella growth.
Defibrinated sheep blood permit the evaluation of hemolytic zone
in grams per liter purified filtered water
|Potato Infusion Solids||
|Defibrinated Sheep Blood||
pH 7,4 +/- 0,2 à 25° C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 30 g in 800 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121° C for 15 minutes. Cool to 45-50° C and aseptically add 200 ml of Defibrinated Sheep Blood (4981). Dispense into sterile Petri dishes.
Prior to inoculate, the prepared medium should be brought to room temperature.
Expected results after 3 days at 35ºC under humid atmosphere
Colonies of Bordetella pertussis are tiny, smooth, with a characteristic compact, glistening appearance.
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 grams||QB-39-0126|
|Defibrinated Sheep Blood||200 ml||4981|
|Plates, 100x15 mm||10/pkg||1810|
|Mono-Plate 100x15 mm||10/pkg||1810M|
|B. Pertussis Transport Medium||10/pkg||2555|
|2325, Dandurand, Suite 300||Phone||Fax||Internet|
|Montreal (Quebec)||(514) 277-2558||(514) 277-4714||http://www.quelab.com|
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