YERSINIA (CIN) SELECTIVE AGAR

Technical Data #1812a / 2013.06.12

CIN is a differential and selective solid medium used for the cultivation and isolation of Yersinia enterocolitica from clinical and nonclinical specimens. Antimicrobial agents, crystal violet and sodium desoxycholate suppress growth of gram-negative and gram- positive organisms.

 


 

FORMULA
in grams per liter purified filtered water

Gelatin Peptone

17,0

Casein Peptone

1,5

Meat Peptone

1,5

Yeast Extract

2,0

D-Mannitol

20,0

Sodium Desoxycholate

0,5

Sodium Cholate

0,5

Sodium Pyruvate

2,0

Magnesium Sulfate

0,01

Sodium Chloride

1,0

Neutral Red

0,03

Crystal Violet

0,001

Irgasan

0,004

Agar

13,5

Supplements

 

Cefsulodin

0,015

Novobiocin

0,0025

pH 7.4 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 59,5 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C and aseptically add 10 ml of Yersinia Supplement (8710). Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plate as soon as possible with specimen and streak for isolation. If inoculation will be delayed, an Enteric Pathogen Transport Medium as EPT-100 should be used.
  2. Incubate aerobically, at 25 C for 24-48 hours.


QUALITY CONTROL

Results after 48 hrs at 25C

Organisms

ATCC

Growth

Yersinia enterocolitica

9610

+

Escherichia coli

25922

- or partial

Proteus mirabilis

12453

- or partial

Enterococcus faecalis

29212

- or partial

Pseudomonas aeruginosa

27853

- or partial

Colonies of Y. enterocolitica, will appear on CIN Agar as deep-red centers, (Bull's eye) surrounded by a transparent border.


LIMITATIONS OF METHOD
Although certain strains of Yersinia can be recovered by direct plating, others may require a cold enrichment (4 C) in phosphate-buffered saline. The CIN Agar is only a part of the identification. Other tests may be required.


REFERENCES

  1. N.C.C.L.S. Quality Assurance for Commercially prepared Microbiological Culture Media. N.C.C.L.S. Publication M-22-A Approved Standard 1990 Vol. 10 No.14.
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Schiemann, D.A. 1979 Synthesis of a selective agar medium for Yersinia enterocolitica. Can. J. Microbiol. 25:1298-1304.
  4. Head, C.B., D.A. Whitty, and S. Ratnam. 1982 Comparative study of selective media for recovery of Yersinia enterocolitica. J. Clin. Microbiol. 16:615-621.
  5. Weissfeld, A.S. and A.C. Sonnenwirth. 1982 Rapid isolation of Yersinia spp. from feces. J. Clin. Microbiol. 15:508-510.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-5614
     
Yersinia Supplement 10x5 ml 8710
     
Prepared media    
Plates, 100x15 mm 10/pkg 1812
     
Enteric Pathogen    
Transport, Vial 100/cs EPT-100

 


 

 

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