QUAD PLATE: ENTEROCOCCI H.L.A.R. (HIGH LEVEL AMINOGLYCOSIDES RESISTANCE)

Technical Data #1851a / 2013.06.15

The Enterococci H.L.A.R. Quad Plate, is used to detect enterococci resistance to high level of aminoglycosides and therapeutic synergy with -lactam positive antibiotics and glycopeptidic antibiotics. Aminoglycosides and glycopeptides may stop an ordinary enterococci infection but can not stop it if there is an highly resistance to aminoglycosides. Streptomycin, gentamycin and vancomycin are added to a Brain Heart Infusion Agar base.

 


 

FORMULA
in grams per liter purified filtered water

Brain Heard Infusion

10,0

Casein/Meat Peptone

17,5

Dextrose

2,0

Sodium Phosphate, dibasic

2,5

Sodium Chloride

5,0

Agar

15,0

 

 

QUAD I: Control X

 

QUAD II: Gentamycin

0,5

QUAD III: Streptomycin

2,0

QUAD IV: Vancomycin

0,006

pH 7,4 +/ 0,2 at 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration).


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Suspend 2-3 similar colonies from a 24 hours growth into a B.H.I. Broth. Adjust turbidity to 0,5 McFarland Standard.
  2. Inoculate each quadrant with 10 l of this dilution. Let it dry.
  3. Incubate at 35 C for 24-48 hours.


QUALITY CONTROL

Results after 24 hrs at 35C

Organisms

ATCC

QUAD I

QUAD II

QUAD III

QUAD IV
Enterococcus faecalis

29212

+

-

-

-

Enterococcus faecalis

49533

+

-

+

+

Enterococcus faecalis

51299

+

+

+

+


LIMITATIONS OF METHOD
Media should be reincubated for 24 hours more to detect a possible resistance. This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Balows, A., Hausler, W.J., Herrmann, K.L., Isenberg, H.D., Shadow, H.J. 1991 Manual of Clinical Microbiology, 5th ed. Washington D.C.: American Society for Microbiology
  2. Sahm, D.F., S. Boonlayangoor, P.C. Iwen, J.L. Baade, G.L. Woods. 1991 Factors influencing determination of High-Level Aminoglycosides resistance in Enterococcus faecalis J. Clin. Microbiol. 29:1934-1039
  3. Sahm, D.F., C. Torres, 1988 Effects of medium and inoculum variations on screening for high-level aminoglycoside resistance of Enterococcus faecalis. J. Clin. Microbiol. 26:250-256
  4. Sahm, D.F., C. Torres 1988 High content aminoglycoside disk for determinating aminoglycoside-penicillin synergy against Enterococcus faecalis. J. Clin. Microbiol. 26:257-260


CATALOGUE NUMBERS

Prepared media    
Plates, 100x15 mm 10/pkg 1851
Mono-Plates, 100x15 mm 10/pkg 1851M
     
BHI Broth    
Tubes, 2ml, 13X100mm 10/pkg 2784

 


 

 

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