BILE ESCULIN AZIDE VANCOMYCIN AGAR

Technical Data #1880a / 2000.11.01

Bile Esculin Azide Vancomycin Agar is a selective screening
medium used for the isolation of esculin hydrolyzing Enterococcus faecalis Vancomycin Resistant.
Sodium azide inhibits gram-negative organisms while bile salts inhibit gram-positive strains other than enterococci and esculin hydrolysis gives black colonies.


FORMULA
in grams per liter purified filtered water

Casein Peptone

16,0

Meat Peptone

9,5

Oxbile

10,0

Esculin

1,0

Sodium Chloride

5,0

Sodium Citrate

1,0

Ferric Ammonium Citrate

0,5

Sodium Azide

0,25

Agar

14,0

Antibiotics

 

Vancomycin

0,006

 

pH 7,1 +/- 0,2 à 25° C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25° C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 57 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121° C for 15 minutes. Cool to 45-50° C. Add 2x5 ml of # 8736 VRE Vancomycin Supplement. Mix thouroughly and dispense into Petri dishes.


PROCEDURE
Bring prepared medium at room temperature before inoculation

  1. Directly, inoculate the specimen and streak for isolated colonies.
  2. Incubate at 35° C for 24-48 hours.


QUALITY CONTROL

Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Esculin Hydrolysis

Enterococcus faecalis

29212

-

-

Enterococcus faecalis

51299

+

black colonies

Enterococcus gallinarum

clinical

+

black colonies


LIMITATIONS OF METHOD
This medium is only a part of the identification.
Other tests may be required.


REFERENCES

  1. Ballows, A., Hausler, W.J.Jr., K.L Herrmann, H.D. Isenberg, Shadomy, H.J. 1991 Manual of Clinical Microbiology. 5th ed. Washington D.C.: American Society for Microbiology.
  2. Sahm, D.F., S. Boonlayangoor, P.C. Iwen, J.L. Baade, G.L. Woods 1991 Factors influencing determination of High-Level Aminoglycoside resistance in Enterococcus faecalis. J. Clin. Microbiol. 29:1934-1939
  3. Swenson, J.M., N.C. Clark, M.J. Ferraro, D.F. Sahm, G. Doern, M.A. Pfaller, L.B. Reller, M.P. Weinstein, R.J. Zabransky, F.C. Tenover 1994 Development of a Standardized Screening Method for Detection of Vancomycin-Resistant Enterococci J. Clin. Microbiol. 32:1700-1704
  4. Tenover, F.C., J. Tokars, J. Swenson, S. Paul, K. Spitalny, W. Jarvis 1993 Ability of clinical laboratories to detect antimicrobial agent-resistant enterococci J. Clin. Microbiol. 31:1695-1699.


CATALOGUE NUMBERS

Dehydrated media

500 grams

QB-39-0118

VRE Vancomycin Supplement

10 vials/pkg

8736

Prepared media

 

Pétri 100x15 mm

10/pkg

1880

Mono-plates, 100x15 mm

10/pkg

1880M


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