m HPC AGAR

Technical Data #1960a / 2013.07.04

m HPC Agar is a solid medium used for the enumeration of heterotrophic microorganisms by membrane filtration method on potable water. Gelatin peptone is a source of nutrients, glycerol is a source of carbohydrates. Microorganisms use gelatin in proteolytic metabolism.

 


 

FORMULA
in grams per liter purified filtered water

Gelatin Peptone

20,0

Gelatin

25,0

Agar

15,0

Glycerol

10,0 ml

pH 7,1 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 60 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Add 10 ml of glycerol (#8467). Sterilize at 121 C for 5 minutes. Cool to 45-50 C. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate surfaces of the plate with membrane filter.
  2. Incubate at 35 C for 48-72 hours.


QUALITY CONTROL

Results after 48 hrs at 35C

Organisms

ATCC

Growth

Enterococcus faecalis

29212

+

Escherichia coli

25922

+

Pseudomonas aeruginosa

27853

+

Pseudomonas aeruginosa

9027

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. LeChevalier, MW. & G.A. McFeters. 1985 Interactions between heterotrophic plate count bacteria and coliforms organisms Appl. Environ. Microbiol. 49:1338
  2. Standards Methods for Examination of Water and Wastewater 1989 17th ed. APHA, Washington D.C.


CATALOGUE NUMBERS

Dehydrated media 500 grams QB-39-2003
Prepared media    
Plate 100x15 mm 10/pkg 1960
Mono-Plate 100x15 mm 10/pkg 1960M

 


 

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