Technical Data #1984a / 2013.06.16
Chocolate Bacitracin Agar is a selective enriched medium for the isolation of Haemophilus sp. Hemoglobin and Bio-X enrichment replace lysed heated blood and give to the culture media enough nutrients agents to permit luxuriant growth of Haemophilus Bacitracin reacts as a selective agent and prevents normal flora growth.
in grams per liter purified filtered water
|Potassium Phosphate, dibasic||
|Potassium Phosphate, monobasic||
pH 7,2 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 36 g of dehydrated media in 500 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Add 500 ml of sterile 2% Hemoglobin (8662), 10 ml of Bio-X Enrichment (8601) and 0,3 g of Bacitracin solution. Mix gently and dispense into sterile Petri dishes.
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 24 hrs at 35ºC W CO2
- or partial
On Chocolate Bacitracin Agar, Haemophilus influenzae colonies are small, colorless, moist and transparent.
LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.
|Dehydrated media||500 g||QB-39-1906|
|Plates, 100x15 mm||10/pkg||1984|
|Mono-Plates, 100x15 mm||10/pkg||1984M|
|Bio-X Enrichment, 10 ml||10/pkg||8601|
|Hemoglobin, 2% sterile||500 ml||8662|
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