P.R.A.S. (PRE-REDUCED ANAEROBIC STERILIZED) TRANSPORT MEDIUM

Technical Data #2017a / 2013.06.16

PRAS Transport Medium is used for collection and transport of anaerobic clinical specimens. Tubes are gassed-out with nitrogen and tighten fitted with butyl stoppers.

 


 

FORMULA
in grams per liter purified filtered water

Sodium Thioglycollate

1,5

Sodium Phosphate, dibasic

0,1

Sodium Chloride

5,0

Calcium Chloride

0,1

Sodium Bisulfite

0,1

L-Cysteine HCl

0,5

Resazurin

0,001

Agar

5,0

pH 8,4 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-25 C protected from direct light.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration).


PROCEDURE

  1. Aseptically, collect specimens. Quickly insert swab or inject fluids through the stopper into medium-containing tube and secure stopper tightly.
  2. Transport immediately to the laboratory.
  3. Subculture specimen into or onto appropriate anaerobes media as soon as possible.
  4. Incubate at 35 C under anaerobic atmosphere for up to 3 days.


QUALITY CONTROL

Subculture after 3 days at 35C reduced O2

Organisms

ATCC

Growth

Bacteroides fragilis

25285

+

Clostridium perfringens

13124

+

Fusobacterium nucleatum

25586

+

Bacteroides levii

29147

+

Peptostreptococcus anaerobius

27337

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Finegold, S.M., E.J. Baron 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby St. Louis
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Prepared media    
Tubes 16x100 mm 10/pkg 2017

 


 





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