MUELLER HINTON BROTH (CATIONS ADJUSTED)

Technical Data #2048a / 2013 .06.19

Mueller Hinton Broth cations adjusted is a liquid medium specially formulated to determine susceptibility as recommended by NCCLS procedures. Appropriate amount of calcium and magnesium provide the correct MIC values when Pseudomonas aeruginosa ATCC 27853 is tested with aminoglycosides antibiotics.

 


 

FORMULA
in grams per liter purified filtered water

Beef Extract

2,0

Casein Hydrolysate, acid

17,5

Starch

1,5

pH 7,3 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 21 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Add 0,05 g of Caclium Chloride and 0,02 g of Magnesium Chloride. Mix thouroughly and dispense into culture tubes. Sterilize at 121 C for 15 minutes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate according to previous standardized procedures.
  2. Incubate at 35 C for 24 hours.
  3. Examine for growth.

MIC of an antibiotic is the lowest concentration which inhibits the growth of inoculated organism.


QUALITY CONTROL

Expected results after 24 hrs at 35C

Organisms

ATCC

NCCLS specifications

Escherichia coli

25922

meets published specifications

Staphylococcus aureus

25923

meets published specifications

Pseudomonas aeruginosa

27853

meets published specifications

Enterococcus faecalis

29212

meets published specifications


LIMITATIONS OF METHOD
All microorganisms found on clinical specimens are not tested with this medium. A control tube without antibiotic should be inoculated with every MIC dilution. This is only a part of the identification. Others tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
  3. NCCLS 1991 Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved Standard M7-A2
  4. Thornsberry, C., T.L. Gavan, E.H. Gerlach, J.C. Sherris 1977 New Developments in Antimicrobial Agent Susceptibility Testing. Cumitech 6, Washington, DC. American Society for Microbiology.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3208
     
Prepared media    
Tubes 16x125 mm, 5 ml 10/pkg 2048
Bottles 8 oz 250 ml 8948

 


 

 

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