MOELLER DECARBOXYLASE BROTH

Technical Data #2061a / 2013.07.02

Moeller Decarboxylase Broths are used for identification of gram- negative by their ability to decarboxylate amino-acids to amine with resultant alkalinity. Pyridoxal enhances decarboxylase reaction but is not required for those microorganisms that exibit a strong decarboxylase activity.

 


 

FORMULA
in grams per liter purified filtered water

Meat Peptone

5,0

Beef Extract

5,0

Dextrose

0,5

Brom Cresol Purple

0,01

Cresol Red

0,005

Pyridoxal HCl

0,005

Specific Amino Acids

10,0

pH 6,0 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 10,5 g of dehydrated media in 900 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Add 100 ml of 10% of each aminoacids solution. To prepare Decarboxylase Control, add 100 ml of purified filtered water. Adjust pH. Dispense into culture tubes. Sterilize at 121 C for 15 minutes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Inoculate the broth by transferring 1 or 2 similar colonies from an overnight growth.
  2. Add 1-2 ml of sterile mineral oil. Tighten cap.
  3. Incubate at 35 C for 24-48 hours. Discard only after 4 days of incubation.


QUALITY CONTROL

Expected results after 24 hrs at 35C

Organisms

ATCC

Lysine

Arginine

Ornithine

Klebsiella pneumoniae

13883

+

-

-

Enterobacter cloacae

23355

-

+

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  3. Washington, J.A. 1981 Laboratory Procedures in Clinical Microbiology Springer-Verlag, New York


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-1120
     
Prepared media    
Tubes, 16x125 mm    
Moeller Decarboxylase Control 10/pkg 2839
Moeller Decarboxylase Lysine 10/pkg 2867
Moeller Decarboxylase Arginine 10/pkg 2061
Moeller Decarboxylase Ornithine 10/pkg 2795

 


 

 

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