STUART TRANSPORT MEDIUM, MODIFIED

Technical Data #2070a / 2013.01.10

This improved medium originally described by Stuart et al., is a non-nutritional semi-solid medium intended for use for the preservation of Neisseria species and other fastidious pathogenic organisms during their transport from collection site to laboratory.

 


 

FORMULA
in grams per liter purified filtered water

Sodium glycerophosphate

10.0

Sodium thioglycollate

0.5

Cysteine hydrochloride

0.5

Calcium chloride

0.1

Methylene Blue

0.001

Agar

5.0

pH 7.4 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only. It must be assumed that all specimens contain infectious micro-organisms; therefore, all specimens should be handle with appropriate precautions. After use, tubes and swabs must be disposed of according to laboratory regulations for infectious waste. Directions for use must be followed carefully.


STORAGE
Store prepared media between 5-25 C protected from direct light. Store dehydrated powder in a dry place in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
A small amount of blue color at the top of the tube indicates oxidation. If this color extends down into the medium it should be discarded. Medium must not be used if there are signs of contamination or if the expiry date has passed. Do not use dehydrated media if it is caked and if expiry date has passed.


DIRECTIONS
Suspend 16 grams of powder in 1 liter of purified filtered water. Mix thoroughly. Heat gently with frequent agitation and boil for one minute. Avoid prolonged heating in open flask because thioglycollate is volatile. Mix carefully and distribute into screw cap culture tubes or bottles. Autoclave for 15 minutes at 121 C (15 psi). Tighten caps immediately after autoclaving.


PROCEDURE

  1. After collection of the specimen, place the swab in the tube, break off the stick, replace the screw cap tightly and transport to the laboratory as soon as possible.
  2. In all cases, specimens should be sub-cultivated onto appropriate primary isolation media as soon as possible or stored in the refrigerator if delay is unavoidable.
  3. Incubate at 35 C for up to 48 hours.


QUALITY CONTROL

Expected results at room temperature after 24 hrs

Organisms

ATCC

Growth

Neisseria gonorrheae

43069

+

Neisseria meningetidis

13090

+

Haemophilus influenzae

19418

+

Pseudomonas aeruginosa

27853

+

Staphylococcus aureus

25923

+

Escherichia coli

25922

+

Streptococcus pyogenes

19615

+

Bacteroides fragilis

25285

+

Bacteroides levii

29147

+

Clostridium difficile

9689

+

Clostridium perfringens

13124

+

Fusobacterium nucleatum

25586

+

Peptostreptococcus anaerobius

27337

+

Trichomonas vaginalis

clinical

+

Uninoculated medium

 

-

All organisms tested remained viable for more than 24 hours when maintained at room temperature.


LIMITATIONS OF METHOD
This transport medium will allow the isolation of gonococci from approximately 90% of cases of gonorrhoea, provided the transport period is under 24 hours. Sodium glycerophosphate may be metabolized by some organisms and thus promote their growth.


REFERENCES

  1. Moffet M., Young. J.L. and Stuart R.D. 1945 British Medical Journal, Vol. 2 , p. 421- 424.
  2. Stuart R.D., Toshach, S.R. and Patsula T.M. 1954 Canad. J. Publ. Hlth., VoL 45, p.13-83.
  3. Stuart, R.D. 1959 Pub. Hlth. Rep. Wash. Vol. 74, p. 431-438


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-5015
     
Prepared media    
Tube 16 x 125 mm, 10 ml 10/pkg 2070

 


 





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