LOEFFLER SERUM MEDIUM

Technical Data #2084a / 2013.06.23

Loeffler Serum Medium is a medium used for isolation and cultivation of Corynebacterium sp. from clinical specimens. Due to the serum content, this medium can be used for proteolytic activity and enzymatic hydrolysis of proteins. The gray-white color is used as background for demonstration of microorganisms pigmentation.

 


 

FORMULA
in grams per liter purified filtered water

Gelatin Peptone

2,5

Beef Extract

1,5

Sodium Chloride

1,25

Dextrose

5,0

Bovine Serum

750 ml

pH 7,6 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration) Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 10,25 g in 250 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Add 750 ml of Bovine Serum (4229). Mix thouroughly and dispense into sterile culture tubes.
Inspissate in a slant position for serum coagulation.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Directly, inoculate with the specimen swab by fishtail motion.
  2. Incubate at 35 C for 24 hours.
  3. Prepare smears and stain with Loeffler Methylene Blue (8569)
  4. Presence of metachromatic granules may be presumptive of Corynebacterium. sp.


QUALITY CONTROL

Expected results after 24 hrs at 35C

Organisms

ATCC

Growth

Corynebacterium diphteriae

11913

+ & metachromatic granules

Staphylococcus aureus

25923

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Buck, T.A., 1949 A modified Loeffler's medium for cultivating Corynebacterium diphteriae. J. Lab. Clin. Med. 34:582-583
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-2615
     
Prepared media    
Tubes, 16x125 mm 10/pk 2084

 


 

 

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