NUTRIENT BROTH

Technical Data #2101a / 2013.08.31

Nutrient Broth is a liquid general purpose medium for the cultivation of non-fastidious microorganisms according to the AOAC and APHA methods.

 


 

FORMULA
in grams per liter purified filtered water

Gelatine Peptone

5,0

Beef Extract

3,0

pH 6,8 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 8 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Dispense into culture tubes and sterilize at 121 C for 15 minutes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate according to APHA or AOAC previous standardized procedures.
  2. Incubate at 35 C for 24-48 hrs with loosened caps.
  3. Examine for growth.


QUALITY CONTROL

Expected results after 24 hrs at 35C

Organisms

ATCC

Growth

Salmonella typhimurium

14028

+

Escherichia coli

25922

+

Staphylococcus aureus

25923

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. AOAC 1999 Official Methods of Analysis of AOAC International 16th, ed
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
  4. Standard Methods for the examination of water and wastewater 1989 17th ed. APHA, washington, D.C.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3506
     
Prepared media    
Tubes 16x125 mm, 5 ml 10/pkg 2101
Tubes 16x125 mm, 10 ml 10/pkg 2801
Bottles w/cap, 300ml, 75cm 10 X 225ml 5289

 


 





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