RAPID UREASE TEST MEDIUM

Technical Data #2171a / 2013.11.01

Rapid Urease Test Medium is used to determine the ability of Helicobacter pylori, formerly Campylobacter pylori, to split urea rapidly by the action of the enzyme urease. The urea substrate is suspended in a monosodium phosphate buffer, and phenol red serves to indicate the pH change that results from urease activity. H. pylori has recently been established as the etiological agent of chronic Active Antral Gastritis (Type B) and may play a role in gastric and duodenal ulcers. H. pylori has a very high endogenous urease activity that has been used for its identification and differentiation from other organisms such as Proteus species, which are capable of splitting urea, but at a much slower rate.

 


 

FORMULA
in grams per liter purified filtered water

Urea

20.0

Monosodium phosphate

0.7

Phenol red

0.1

Agar

4.0

pH 6.4 +/- 0.2 at 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
The prepared media should be stored at 2-8C away from direct light.


SIGN OF DETERIORATION
The media must not be used if there are signs of contamination or deterioration or if expiry date has passed.

SPECIMEN COLLECTION
Biopsy specimens are placed directly into the Rapid Urease Test Medium at the time of endoscopy.


METHOD OF USE
Submerge the biopsy specimen in the medium. Incubate the inoculated tube aerobically at room temperature for up to 20 hours to 3 days. A strong positive reaction may be noted within minutes of inoculation.


INTERPRETATION
POSITIVE: Intense Pink-Red (Red-Violet) color.
NEGATIVE: No color change


QUALITY CONTROL

Expected results

Organisms

ATCC

Growth

Helicobacter pylori

43504

+

Proteus mirabilis

12453

+
(slow reaction time)

Escherichia coli

25922

-


LIMITATIONS OF METHOD
Inoculating loops made of nichrome generate an immediate, spuriously positive test result; a platinum or plastic loop or wooden stick should be used. This medium is only part of the identification. Other tests may be required.


REFERENCES

  1. Barthel, L., et al, 1986. The predictive value of a simple rapid urease test for Campylobacter pyloridis in asymptomatic volunteers. Am. J. Gastroenterology. 81:852
  2. Hazell, S.L., et al, 1987. Campylobacter pyloridis gastritis. I. Detection of urease as a marker of bacterial colonization and gastritis. Am.J. Gastroenterology. 82:292-296
  3. Goldie, J. et al, 1989. Optimization of a medium for the Rapid Urease Test for detection of Campylobacter pylori in gastric antral biopsies. J. of Clin. Microbiol., 27: 2080-2082
  4. Pique, J.M., et al, 1989. Rapid Detection of gastric Campylobacter pylori colonization by a simple biochemical test. J. of Clin. Microbiol., 27: 2604-2605
  5. Goldie, J. et al, 1989. Media for the culture of Campylobacter pylori. Am.J. Gastroenterology, 84:79


CATALOGUE NUMBERS

Rapid Urease Test Medium, 10 x 2.0 ml 17 x 60 mm vial 2171

 


 

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