Technical Data #2290a / 2013.09.26
Sabouraud Dextrose Broth is a liquid medium used for cultivation of yeasts and molds from liquid which are normally sterile. Acid pH of the medium inhibits bacterial growth and enhances yeasts and molds recovery.
in grams per liter purified filtered water
|Casein and Meat Peptone||
pH 5,6 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 30 g of dehydrated media in 1000 ml of purified filtered water. Heat to boil for one minute. Mix gently and dispense into culture tubes. Sterilize at 121 C for 15 minutes.
Prior to inoculate, the prepared media should be brought to room temperature.
Results after 3 days at 25ºC
- or partial
LIMITATIONS OF METHOD
This is only a part of the identification. Other tests may be required.
|Dehydrated media||500 grams||QB-39-3816|
|Tubes 16x125 mm, 5 ml||10/pkg||2924|
|Tubes 20x125 mm, 15 ml||10/pkg||2290|
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