SABOURAUD DEXTROSE BROTH

Technical Data #2290a / 2013.09.26

Sabouraud Dextrose Broth is a liquid medium used for cultivation of yeasts and molds from liquid which are normally sterile. Acid pH of the medium inhibits bacterial growth and enhances yeasts and molds recovery.

 


 

FORMULA
in grams per liter purified filtered water

Casein and Meat Peptone

10,0

Dextrose

20,0

pH 5,6 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 30 g of dehydrated media in 1000 ml of purified filtered water. Heat to boil for one minute. Mix gently and dispense into culture tubes. Sterilize at 121 C for 15 minutes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Inoculate tubes with specimens.
  2. Incubate at 25 C for 3 days and than up to 7 days.
  3. Subculture on a general purpose for yeasts and molds culture media as a Sabouraud Dextrose agar (#1015).


QUALITY CONTROL

Results after 3 days at 25C

Organisms

ATCC

Growth

Trichophyton mentagrophytes

9533

+

Candida albicans

10231

+

Escherichia coli

25922

- or partial


LIMITATIONS OF METHOD
This is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J. Jr, and Shadomy, H.J. 1985 Manual of Clinical Microbiology, 4th ed., Washington, DC. American Society for Microbiology.
  2. Mac Faddin, Jean, F. 1985 Identification-Cultivation-Maintenance of Medical Bacteria Vol.1, Baltimore, MD, Williams & Wilkins
  3. N.C.C.L.S. 1996 Quality Assurance for Commercially Prepared Microbiological Culture Media Approved Standard. N.C.C.L.S. Document M22-A2 Vol.16, No. 16


CATALOGUE NUMBERS

Dehydrated media 500 grams QB-39-3816
     
Prepared media    
Tubes 16x125 mm, 5 ml 10/pkg 2924
Tubes 20x125 mm, 15 ml 10/pkg 2290

 


 

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