T.S.B. (TRYPTIC SOY) BROTH

Technical Data #2341a / 2013.03.06

TSB Broth is a liquid general purpose medium for the cultivation of non-fastidious and fastidious microorganisms with addition of some enrichments.

 


 

FORMULA
in grams per liter purified filtered water

Casein Peptone

17,0

Soy Peptone

3,0

Dextrose

2,5

Sodium Chloride

5,0

Potassium Phosphate, dibasic

2,5

pH 7.3 +/- 0,2 25 C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25 C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 30 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Mix thouroughly and dispense into sterile culture tubes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate according to previous standardized procedures.
  2. Incubate at 35 C for 24-48 heures with loosened caps.
  3. Examine for growth.


QUALITY CONTROL

Expected results after 24 hrs at 35C

Organisms

ATCC

Growth

Escherichia coli

25922

+

Staphylococcus aureus

25923

+

Candida albicans

10231

+

Bacillus subtilis

6633

+

Streptococcus pneumoniae

6305

+

Streptococcus pyogenes

19615

+


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
  3. NCCLS 1990 Performance standard for antimicrobial disc susceptibility tests Approved Standard. Document:M2-A4 Vol. 10, No. 7
  4. Standard Methods for the examination of water and wastewater 1989 17th ed. APHA, washington, D.C.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-5206
     
Prepared media    
Tubes    
13x100 mm, 2 ml 10/pkg 2341
13x100 mm, 0,5 ml 10/pkg 2750
16x125 mm, 5 ml 10/pkg 2395
16x125 mm, 10 ml 10/pkg 2569
20x125 mm, 15 ml 10/pkg 2648
20x125 mm, 20 ml 10/pkg 2828
Bottles 3 oz 80 ml 8901
Bottles 1 L 500 ml 8905

 


 

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