SALINE 0,85% SOLUTION (PHYSIOLOGICAL WATER)

Technical Data #2350a / 2013.06.25

Saline 0,85% is a liquid solution used for the preparation of microbial suspension for subculturing, for disk susceptibility testing or for identification test battery.

 


 

FORMULA
in grams per liter purified filtered water

Sodium Chloride

8,5


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8 C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25 C.

 

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 8,5 g in 1000 ml of purified water. Dissolve on vortex. Sterilize at 121 C for 15 minutes. Cool to 45-50 C. Mix thouroughly and dispense into sterile culture tubes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Inoculate according to previous standardized procedures.
  2. Use as required.


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Finegold, S.M., E.J. Baron 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby St. Louis
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. NCCLS 1990 Performance standard for antimicrobial disc susceptibility tests Approved Standard. Document:M2-A4 Vol. 10, No. 7
  4. NCCLS 1990 Quality Assurance for Commercially Prepared Microbiological Culture Media Approved Standard Document M22-A Vol.10, No.14


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-4109
     
Prepared media    
Tubes 16x125 mm, 5 ml 10/pkg 2350
  100/box 2350-H

 


 

 

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