M.I.O. (MOTILITY INDOLE ORNITHINE) MEDIUM

Technical Data #2360a / 2013.11.01

MIO Test Medium is a semisolid medium used as differential test medium by ability to produce indole from tryptophan, to decarboxylate the ornithine and exibit motility by turbidity throughout of medium.

FORMULA
in grams per liter purified filtered water

Gelatin Peptone	10,0
Casein Peptone	10,0
Yeast Extract	3,0
Dextrose	1,0
Bromcresol Purple	0,02
L-Ornithine	5,0
Agar	2,0

pH 6,5 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25° C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 31 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Mix thouroughly and dispense into culture tubes. Sterilize at 121° C for 15 minutes.

PROCEDURE
Bring prepared medium at room temperature before inoculation.

1. Inoculate with 2-3 similar colonies from an overnight growth, by stabbing with a straight needle to over half the depth.
2. Incubate at 35° C for 24 hours with loosened caps.
3. Examine for motility, for ornithine decarboxylate. Add 2-3 drops of Kovac's Reagent (8552), observe for production of red ring (positive reaction for indole).

QUALITY CONTROL
Expected results after 24 hrs at 35ºC

Organisms	ATCC	Motility	Indole	Ornithine
Escherichia coli	25922	+	+	+
Enterobacter aerogenes	13028	+	-	+
Klebsiella pneumoniae	13883	-	-	-

LIMITATIONS OF METHOD
This is only a part of the identification. Other tests may be required.

REFERENCES

1. Ewing, W.H., 1986 Edwards and Ewing's identification of Enterobacteriaceae 4th ed. Elsevier Science Publishing Co. New York
2. Finegold, S.M., E.J. Baron 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby St. Louis
3. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
4. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.

CATALOGUE NUMBERS

Dehydrated media	500 grams	QB-39-3433
Kovac's Reagent	30 ml	8552
	100 ml	8581
Prepared media
Tubes 16x125 mm, 5 ml	10/pkg	2360