Technical Data #2360a / 2013.11.01
MIO Test Medium is a semisolid medium used as differential test medium by ability to produce indole from tryptophan, to decarboxylate the ornithine and exibit motility by turbidity throughout of medium.
FORMULA
in grams per liter purified filtered water
Gelatin Peptone |
10,0 |
Casein Peptone |
10,0 |
Yeast Extract |
3,0 |
Dextrose |
1,0 |
Bromcresol Purple |
0,02 |
L-Ornithine |
5,0 |
Agar |
2,0 |
pH 6,5 +/- 0,2 à 25º C |
This approximate formula may be adjusted and/or enriched to obtain best results.
PRECAUTIONS
This medium is for IN VITRO diagnostic use only.
STORAGE
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25° C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.
DIRECTIONS
Suspend 31 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Mix thouroughly and dispense into culture tubes. Sterilize at 121° C for 15 minutes.
PROCEDURE
Bring prepared medium at room temperature before inoculation.
QUALITY CONTROL
Expected results after 24 hrs at 35ºC
Organisms |
ATCC |
Motility |
Indole |
Ornithine |
Escherichia coli |
25922 |
+ |
+ |
+ |
Enterobacter aerogenes |
13028 |
+ |
- |
+ |
Klebsiella pneumoniae |
13883 |
- |
- |
- |
LIMITATIONS OF METHOD
This is only a part of the identification. Other tests may be required.
REFERENCES
CATALOGUE NUMBERS
Dehydrated media | 500 grams | QB-39-3433 |
Kovac's Reagent | 30 ml | 8552 |
100 ml | 8581 | |
Prepared media | ||
Tubes 16x125 mm, 5 ml | 10/pkg | 2360 |
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